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Balbuena,Tiago Santana; Santa-Catarina,Claudete; Silveira,Vanildo; Kato,Massuo Jorge; Floh,Eny Iochevet Segal. |
Piper solmsianum is a shrub from Southeast Brazil in which many biologically active compounds were identified. The aim of this work was to establish a cell suspension culture system for this species. With this in mind, petiole and leaf explants obtained from in vitro plantlets were cultured in the presence of different plant growth regulator combinations (IAA, NAA, 2,4-D and BA). Root and indirect shoot adventitious formation, detected by histological analysis, was observed. Besides the different combinations of plant growth regulators, light regime and the supplement of activated charcoal (1.5 mg.l-1) were tested for callus induction and growth. Cultures maintained in light, on a 0.2 mg.l-1 2,4-D and 2 mg.l-1 BA supplemented medium, and in the absence of... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Callogenesis; Cell suspension culture; Morphogenesis; Pariparoba; Plant growth regulators. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062009000100029 |
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